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Journal: STAR Protocols

Article Title: Assessment of escape from X chromosome inactivation and gene expression in single human immune cells

doi: 10.1016/j.xpro.2021.100641

Figure Lengend Snippet:

Article Snippet: Refer to p. 27ff of the Juno System user guide (PN 100–7070 r12) on how to change the interface plate: https://www.fluidigm.com/binaries/content/documents/fluidigm/resources/juno-user-guide-ug-100-7070/juno-user-guide-ug-100-7070/fluidigm%3Afile After pipetting of all the samples, assays and pressure fluid, place the 192.24 IFC into the Juno and run the following script: Load Mix 192.24 GT.

Techniques: Recombinant, Suspension, Saline, Binding Assay, Control, Multiplex Assay, Cell Isolation, Real-time Polymerase Chain Reaction, Software, Gene Expression, Transferring, Fluorescence, Microscopy